Journal: bioRxiv

Article Title: Auranofin potentiates cisplatin response through context-dependent NOTCH-associated signaling states in endometrial cancer

doi: 10.64898/2026.05.27.728338

Figure Lengend Snippet: (A) Kaplan–Meier analysis of overall survival in the TCGA-UCEC cohort stratified by NOTCH3 copy-number using upper-versus-lower quartile grouping. Tumors with elevated NOTCH3 copy-number exhibited significantly reduced overall survival compared with tumors harboring low copy-number. (B) Kaplan–Meier analysis of overall survival stratified by NOTCH3 RNA expression using upper-versus-lower quartile grouping. NOTCH3 RNA expression demonstrated only a modest, nonsignificant association with outcome. (C) Kaplan–Meier analysis of overall survival according to NOTCH3 mutation status. No significant association between NOTCH3 mutation status and overall survival was observed. Log-rank P values are indicated on each panel. Tables beneath Kaplan–Meier plots indicate the number of patients at risk at the indicated time points (days).

Article Snippet: Rabbit polyclonal anti-NOTCH3 antibody was from ABclonal Technology (Cat# A13522, RRID:AB_2760384; 1:2,000).

Techniques: Low Copy Number, RNA Expression, Mutagenesis

Journal: bioRxiv

Article Title: Auranofin potentiates cisplatin response through context-dependent NOTCH-associated signaling states in endometrial cancer

doi: 10.64898/2026.05.27.728338

Figure Lengend Snippet: (A) Western blot analysis of full-length NOTCH3 (NOTCH3-FL) and activated NOTCH3 intracellular domain (NICD3) in EC cell lines. GAPDH served as a loading control. (B) Summary of AuR and CDDP IC50 values across EC cell lines. IC50 values are presented as mean ± SEM. NICD3 abundance was categorized qualitatively based on immunoblot analysis shown in panel A. (C) Dose-response analysis of AuR sensitivity in EC cell lines. (D) Dose-response analysis of CDDP sensitivity in EC cell lines. Dose-response curves in panels C and D were fitted using a four-parameter logistic regression model.

Article Snippet: Rabbit polyclonal anti-NOTCH3 antibody was from ABclonal Technology (Cat# A13522, RRID:AB_2760384; 1:2,000).

Techniques: Western Blot, Control

Journal: bioRxiv

Article Title: Auranofin potentiates cisplatin response through context-dependent NOTCH-associated signaling states in endometrial cancer

doi: 10.64898/2026.05.27.728338

Figure Lengend Snippet: (A) Schematic model illustrating canonical NOTCH3 signaling and the proposed mechanism of AuR action. Following ligand-dependent NOTCH3 activation, NICD3 forms a transcriptional activation complex with RBPJ at canonical NOTCH-responsive promoters, including HES1 and HES4 . AuR reduces RBPJ occupancy at canonical NOTCH-responsive promoters. (B–D) Representative RBPJ chromatin immunoprecipitation (ChIP) assays in RL95-2 (B), ARK-1 (C), and AN3CA (D) cells following treatment with DMSO or 2.5 µM AuR. RBPJ occupancy at the HES1 and HES4 loci was measured by qPCR and normalized to input chromatin. Bead-only controls were included to assess nonspecific background enrichment. (E) Representative nonlinear regression curves from one experiment showing CDDP response in AN3CA cells treated with increasing concentrations of CDDP in the absence or presence of 0.75 or 1.0 µM AuR. Relative proliferation was normalized to untreated controls. (F) Replicate-level quantification of relative CDDP IC50 values from independent biological replicates across AuR concentrations, including 0.75, 1.0, and 1.5 µM AuR conditions. IC50 values were normalized to the no-AuR condition. Data are presented as mean ± SEM. Statistical significance in panel F was determined using one-way ANOVA with multiple-comparison correction. ns, not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001.

Article Snippet: Rabbit polyclonal anti-NOTCH3 antibody was from ABclonal Technology (Cat# A13522, RRID:AB_2760384; 1:2,000).

Techniques: Activation Assay, Chromatin Immunoprecipitation, Comparison

Journal: bioRxiv

Article Title: Auranofin potentiates cisplatin response through context-dependent NOTCH-associated signaling states in endometrial cancer

doi: 10.64898/2026.05.27.728338

Figure Lengend Snippet: (A) Western blot validation of NOTCH3 knockdown in AN3CA, KLE, and ARK-1 cells using independent shRNAs targeting NOTCH3 (3A and 3C). GAPDH served as a loading control. (B–D) Dose-response analysis of AuR response in Ctrl KD, NOTCH3A KD, and NOTCH3C KD cells. Representative nonlinear regression curves are shown at left, with replicate-level area under the curve (AUC) quantification shown at right. (E) Dose-response analysis of CDDP response in AN3CA cells following NOTCH3 depletion. Representative nonlinear regression curves are shown at left, with replicate-level AUC quantification shown at right. Relative proliferation values were normalized to vehicle-treated controls. Data are presented as mean ± SEM. AUC values were calculated from replicate dose-response curves. Statistical significance was determined using one-way ANOVA with multiple-comparison correction. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.

Article Snippet: Rabbit polyclonal anti-NOTCH3 antibody was from ABclonal Technology (Cat# A13522, RRID:AB_2760384; 1:2,000).

Techniques: Western Blot, Biomarker Discovery, Knockdown, Control, Comparison

Journal: Bosnian Journal of Basic Medical Sciences

Article Title: The association between NOTCH3 expression and the clinical outcome in the urothelial bladder cancer patients

doi: 10.17305/bjbms.2021.6767

Figure Lengend Snippet: NOTCH3 expression in UBC; (A) NOTCH3-positive endothelial cells and NOTCH3-negative (0) tumor cells in pTa UBC (×100); (B) intermediate, focal NOTCH3 (1) staining in high-grade pT1 UBC (×200); (C) intermediate, diffuse NOTCH3 (2) staining in low-grade pT1 UBC (×100); (D) intense, diffuse NOTCH3 (3) staining in pT2 UBC (×200). UBC: Urothelial bladder cancer

Article Snippet: After preparation, the slides were processed in a semi-automatic IHC diagnostic system (Ventana Inc.) and the IHC staining was performed using a rabbit polyclonal antibody to NOTCH3 (ab23426, Abcam, Cambridge, UK) at a concentration of 5 mg/ml.

Techniques: Expressing, Staining

Journal: Bosnian Journal of Basic Medical Sciences

Article Title: The association between NOTCH3 expression and the clinical outcome in the urothelial bladder cancer patients

doi: 10.17305/bjbms.2021.6767

Figure Lengend Snippet: Histopathological and clinical characteristics of urothelial bladder cancer patients according to NOTCH3 expression

Article Snippet: After preparation, the slides were processed in a semi-automatic IHC diagnostic system (Ventana Inc.) and the IHC staining was performed using a rabbit polyclonal antibody to NOTCH3 (ab23426, Abcam, Cambridge, UK) at a concentration of 5 mg/ml.

Techniques: Expressing

Journal: Bosnian Journal of Basic Medical Sciences

Article Title: The association between NOTCH3 expression and the clinical outcome in the urothelial bladder cancer patients

doi: 10.17305/bjbms.2021.6767

Figure Lengend Snippet: Kaplan–Meier survival curves showing overall survival of 614 bladder cancer patients with negative (0=no staining; 1=weak and/or focal staining) and positive (2=diffuse, intermediate staining; 3=strong, diffuse staining) NOTCH3 expression.

Article Snippet: After preparation, the slides were processed in a semi-automatic IHC diagnostic system (Ventana Inc.) and the IHC staining was performed using a rabbit polyclonal antibody to NOTCH3 (ab23426, Abcam, Cambridge, UK) at a concentration of 5 mg/ml.

Techniques: Staining, Expressing

Journal: Bosnian Journal of Basic Medical Sciences

Article Title: The association between NOTCH3 expression and the clinical outcome in the urothelial bladder cancer patients

doi: 10.17305/bjbms.2021.6767

Figure Lengend Snippet: Kaplan–Meier survival curves showing cancer-specific survival of 614 bladder cancer patients with negative (0=no staining; 1=weak and/or focal staining) and positive (2= intermediate, diffuse staining; 3=strong, diffuse staining) NOTCH3 expression.

Article Snippet: After preparation, the slides were processed in a semi-automatic IHC diagnostic system (Ventana Inc.) and the IHC staining was performed using a rabbit polyclonal antibody to NOTCH3 (ab23426, Abcam, Cambridge, UK) at a concentration of 5 mg/ml.

Techniques: Staining, Expressing

Journal: Bosnian Journal of Basic Medical Sciences

Article Title: The association between NOTCH3 expression and the clinical outcome in the urothelial bladder cancer patients

doi: 10.17305/bjbms.2021.6767

Figure Lengend Snippet: Kaplan–Meier survival curves showing recurrence-free survival of 614 bladder cancer patients with negative (0=no staining; 1=weak and/or focal staining) and positive (2=intermediate, diffuse staining; 3=strong, diffuse staining) NOTCH3 expression.

Article Snippet: After preparation, the slides were processed in a semi-automatic IHC diagnostic system (Ventana Inc.) and the IHC staining was performed using a rabbit polyclonal antibody to NOTCH3 (ab23426, Abcam, Cambridge, UK) at a concentration of 5 mg/ml.

Techniques: Staining, Expressing

Journal: International Journal of Oncology

Article Title: Regulation of gliomagenesis and stemness through acid sensor ASIC1a

doi: 10.3892/ijo.2021.5262

Figure Lengend Snippet: Notch signaling is negatively associated with ASIC1a in glioblastoma multiforme. (A-C) The glioma cells were transfected with shASIC1a and control followed by assaying protein expression of ASIC1a, active form of Notch1, Notch2, Notch3, Notch4, and Notch target survivin, as well as GSC markers CD133 and ALDH1 by western blotting in (A) A172, (B) U87MG, and (C) PDX-L12 cells. (D-F) Subsequently, the glioma cells (D) A172, (E) U87MG and (F) PDX-L12 were transfected with ASIC1a-FLAG followed by assaying protein expression of ASIC1a, active form of Notch1, Notch2, Notch3, Notch4 and Notch target survivin, as well as GSC markers CD133 and ALDH1 by western blotting. * P<0.05 and ** P<0.01. ASIC1a, acid-sensing ion channel 1a; sh, short hairpin; GSC, glioblastoma stem cells; ALDH1, aldehyde dehydrogenase 1.

Article Snippet: Rabbit polyclonal anti-Notch3 antibody (product code ab60087) was purchased from Abcam.

Techniques: Transfection, Control, Expressing, Western Blot